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cd47 fitc  (Miltenyi Biotec)


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    Miltenyi Biotec cd47 fitc
    Cd47 Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd47+fitc/pm41776652-101-16-27?v=Miltenyi+Biotec
    Average 94 stars, based on 11 article reviews
    cd47 fitc - by Bioz Stars, 2026-07
    94/100 stars

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    Infection with XVir-N-31 (XVir) increases both calreticulin (CALR) and <t>CD47</t> surface expression on pediatric sarcoma cell lines. (A) Analysis of CALR (‘eat-me’) and CD47 surface expression (‘don’t-eat-me’) surface expression of pediatric sarcoma cell lines A673, SKNMC, and U2OS 48 hours post infection (hpi) at indicated multiplicity of infection (MOI) assessed by FACs analysis after dead cell exclusion via DAPI. Y-axis depicts the fold change of expression compared to controls (ctrl) using frequency of parent minus isotype (IT). (B) Analysis MOI/dose-dependency of CD47 surface expression at 48hpi using indicated MOI. Statistical analysis was performed using the unpaired student’s t-test in (A) and one way ANOVA with multiple comparison and Tukey correction in (B) . Plotted is the mean with SD. Each dot represents one biological replicate. Experiments were repeated at least twice to ensure reproducibility. Levels of significance are indicated as asterisks *p<0,0332; **p<0,0021; ***p<0,0002; ****p<0,0001.
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    Infection with XVir-N-31 (XVir) increases both calreticulin (CALR) and <t>CD47</t> surface expression on pediatric sarcoma cell lines. (A) Analysis of CALR (‘eat-me’) and CD47 surface expression (‘don’t-eat-me’) surface expression of pediatric sarcoma cell lines A673, SKNMC, and U2OS 48 hours post infection (hpi) at indicated multiplicity of infection (MOI) assessed by FACs analysis after dead cell exclusion via DAPI. Y-axis depicts the fold change of expression compared to controls (ctrl) using frequency of parent minus isotype (IT). (B) Analysis MOI/dose-dependency of CD47 surface expression at 48hpi using indicated MOI. Statistical analysis was performed using the unpaired student’s t-test in (A) and one way ANOVA with multiple comparison and Tukey correction in (B) . Plotted is the mean with SD. Each dot represents one biological replicate. Experiments were repeated at least twice to ensure reproducibility. Levels of significance are indicated as asterisks *p<0,0332; **p<0,0021; ***p<0,0002; ****p<0,0001.
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    Image Search Results


    Antibodies used for flow cytometry, immunocytochemistry, and immunohistochemistry.

    Journal: Frontiers in Genome Editing

    Article Title: CRISPR-Cas9 immune-evasive hESCs are rejected following transplantation into immunocompetent mice

    doi: 10.3389/fgeed.2024.1403395

    Figure Lengend Snippet: Antibodies used for flow cytometry, immunocytochemistry, and immunohistochemistry.

    Article Snippet: Rat anti-mouse CD47 − FITC, Monoclonal Antibody (miap301) , 1:40 , Thermo Fisher Scientific Cat# 11-0471-82.

    Techniques: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Marker, Recombinant, Control, Knock-Out, Staining, Immunohistochemical staining

    Infection with XVir-N-31 (XVir) increases both calreticulin (CALR) and CD47 surface expression on pediatric sarcoma cell lines. (A) Analysis of CALR (‘eat-me’) and CD47 surface expression (‘don’t-eat-me’) surface expression of pediatric sarcoma cell lines A673, SKNMC, and U2OS 48 hours post infection (hpi) at indicated multiplicity of infection (MOI) assessed by FACs analysis after dead cell exclusion via DAPI. Y-axis depicts the fold change of expression compared to controls (ctrl) using frequency of parent minus isotype (IT). (B) Analysis MOI/dose-dependency of CD47 surface expression at 48hpi using indicated MOI. Statistical analysis was performed using the unpaired student’s t-test in (A) and one way ANOVA with multiple comparison and Tukey correction in (B) . Plotted is the mean with SD. Each dot represents one biological replicate. Experiments were repeated at least twice to ensure reproducibility. Levels of significance are indicated as asterisks *p<0,0332; **p<0,0021; ***p<0,0002; ****p<0,0001.

    Journal: Frontiers in Oncology

    Article Title: YB-1-based oncolytic virotherapy in combination with CD47 blockade enhances phagocytosis of pediatric sarcoma cells

    doi: 10.3389/fonc.2024.1304374

    Figure Lengend Snippet: Infection with XVir-N-31 (XVir) increases both calreticulin (CALR) and CD47 surface expression on pediatric sarcoma cell lines. (A) Analysis of CALR (‘eat-me’) and CD47 surface expression (‘don’t-eat-me’) surface expression of pediatric sarcoma cell lines A673, SKNMC, and U2OS 48 hours post infection (hpi) at indicated multiplicity of infection (MOI) assessed by FACs analysis after dead cell exclusion via DAPI. Y-axis depicts the fold change of expression compared to controls (ctrl) using frequency of parent minus isotype (IT). (B) Analysis MOI/dose-dependency of CD47 surface expression at 48hpi using indicated MOI. Statistical analysis was performed using the unpaired student’s t-test in (A) and one way ANOVA with multiple comparison and Tukey correction in (B) . Plotted is the mean with SD. Each dot represents one biological replicate. Experiments were repeated at least twice to ensure reproducibility. Levels of significance are indicated as asterisks *p<0,0332; **p<0,0021; ***p<0,0002; ****p<0,0001.

    Article Snippet: Following FACS-antibodies were used: CD47 anti-human FITC REA 220 (Miltenyi Biotech), CD47 isotype REA control antibody human IgG1 VioBright B515 REA293 (Miltenyi Biotech), Calreticulin mouse anti-human Alexa Fluor 488 MAB38981 (R&D Systems), Alexa Fluor 488 goat anti-mouse IgG A11029 (Thermo Fisher Scientific), CD11c anti-human APC B-ly6 (BD Biosciences) and CD45 anti-human APC REA747 (Miltenyi Biotech), CD86 anti-human VioBlue REA968 (Miltenyi Biotech), CD80 anti-human PE REA661 (DAPI Staining Solution (Miltenyi Biotec).

    Techniques: Infection, Expressing, Comparison

    The combination (combo) of XVir-N-31 (XVir) and the CD47-inhbitor (CD47i) B6H12.2 shows the highest levels of phagocytosis for all tested phagocytes and cell lines. Phagocytosis by THP-1 macrophages (A) , THP-1 imDCs (B) , and healthy donor-derived monocytic imDCs (C) was assessed at indicated MOI (48hpi) and time (y-axis) for A673 (left panels) and U2OS (right panels). CD47i was added when starting phagocytosis. Each dot represents one biological replicate. Experiments were repeated at least three times. Plotted is the normalized phagocytosis compared to ctrl as mean and SD. One way ANOVA with multiple comparison and Tukey correction was used for statistical analysis. Levels of significance are indicated as asterisks *p<0,0332; **p<0,0021; ***p<0,0002; ****p<0,0001.

    Journal: Frontiers in Oncology

    Article Title: YB-1-based oncolytic virotherapy in combination with CD47 blockade enhances phagocytosis of pediatric sarcoma cells

    doi: 10.3389/fonc.2024.1304374

    Figure Lengend Snippet: The combination (combo) of XVir-N-31 (XVir) and the CD47-inhbitor (CD47i) B6H12.2 shows the highest levels of phagocytosis for all tested phagocytes and cell lines. Phagocytosis by THP-1 macrophages (A) , THP-1 imDCs (B) , and healthy donor-derived monocytic imDCs (C) was assessed at indicated MOI (48hpi) and time (y-axis) for A673 (left panels) and U2OS (right panels). CD47i was added when starting phagocytosis. Each dot represents one biological replicate. Experiments were repeated at least three times. Plotted is the normalized phagocytosis compared to ctrl as mean and SD. One way ANOVA with multiple comparison and Tukey correction was used for statistical analysis. Levels of significance are indicated as asterisks *p<0,0332; **p<0,0021; ***p<0,0002; ****p<0,0001.

    Article Snippet: Following FACS-antibodies were used: CD47 anti-human FITC REA 220 (Miltenyi Biotech), CD47 isotype REA control antibody human IgG1 VioBright B515 REA293 (Miltenyi Biotech), Calreticulin mouse anti-human Alexa Fluor 488 MAB38981 (R&D Systems), Alexa Fluor 488 goat anti-mouse IgG A11029 (Thermo Fisher Scientific), CD11c anti-human APC B-ly6 (BD Biosciences) and CD45 anti-human APC REA747 (Miltenyi Biotech), CD86 anti-human VioBlue REA968 (Miltenyi Biotech), CD80 anti-human PE REA661 (DAPI Staining Solution (Miltenyi Biotec).

    Techniques: Derivative Assay, Comparison